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pet 28a expression vectors  (Twist Bioscience)

 
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    Twist Bioscience pet 28a expression vectors
    Pet 28a Expression Vectors, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+28a+expression+vectors/bio_rxiv__2025__11__27__690864-94-19-23?v=Twist+Bioscience
    Average 86 stars, based on 1 article reviews
    pet 28a expression vectors - by Bioz Stars, 2026-08
    86/100 stars

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    (A) In silico cloning of the vaccine construct sequence into the <t>pET-28a</t> (+) expression vector. The construct sequence (blue) was cloned between the vector's EcoRI and BamHI restriction sites (black). (B) 1% agarose gel electrophoresis simulation. The lanes show the molecular weight (MW), and the cloning results in positions 1–3, where lane 1, lane 2, and lane 3 represent the vaccine construct (888bp), the pET-28a (+) (5369bp), and the recombinant plasmid (6245bp), respectively.
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    (A) In silico cloning of the vaccine construct sequence into the pET-28a (+) expression vector. The construct sequence (blue) was cloned between the vector's EcoRI and BamHI restriction sites (black). (B) 1% agarose gel electrophoresis simulation. The lanes show the molecular weight (MW), and the cloning results in positions 1–3, where lane 1, lane 2, and lane 3 represent the vaccine construct (888bp), the pET-28a (+) (5369bp), and the recombinant plasmid (6245bp), respectively.

    Journal: Journal of Immunology Research

    Article Title: In Silico Design and Characterization of a Multiepitope Vaccine Candidate Against Brucella canis Using a Reverse Vaccinology Approach

    doi: 10.1155/jimr/6348238

    Figure Lengend Snippet: (A) In silico cloning of the vaccine construct sequence into the pET-28a (+) expression vector. The construct sequence (blue) was cloned between the vector's EcoRI and BamHI restriction sites (black). (B) 1% agarose gel electrophoresis simulation. The lanes show the molecular weight (MW), and the cloning results in positions 1–3, where lane 1, lane 2, and lane 3 represent the vaccine construct (888bp), the pET-28a (+) (5369bp), and the recombinant plasmid (6245bp), respectively.

    Article Snippet: The pET-28a (+) expression vector, obtained from the Addgene database ( https://www.addgene.org/ ) [ ], was selected as the cloning vector.

    Techniques: In Silico, Cloning, Construct, Sequencing, Expressing, Plasmid Preparation, Clone Assay, Agarose Gel Electrophoresis, Molecular Weight, Recombinant